arthur.tugume@mak.ac.ug; aktugume@gmail.com; tugumeka@yahoo.com
+256 772514841, +256 704514841
School of Biosciences in the College of Natural Sciences, Makerere University

Assoc. Prof. Arthur K. Tugume, PhD

Associate Professor, Dean, School of Biosciences in the College of Natural Sciences, Makerere University

Research Interest:

Biography

Dr. Arthur Tugume is a Ugandan scientist based at Makerere University, Uganda. He is an Associate Professor of Plant Pathology and Virology at the Department of Plant Sciences, Microbiology and Biotechnology, where he has served in various academic, administrative, and management capacities since 1999. He is a two-time Head and Chair of the Department of Plant Sciences, Microbiology and Biotechnology (2012-2015 and 2020-2022). He is currently the Dean, School of Biosciences in the College of Natural Sciences, Makerere University (2022-2026).

Dr. Tugume holds a PhD in Plant Pathology from the University of Helsinki, Finland (2010). He also holds an M.Sc in Agriculture (Crop Science) (2003) and B.Sc (Botany and Biochemistry) (1999) both from Makerere University. Dr. Tugume is involved in scientific research with collaborative networks in Uganda, Kenya, Tanzania, Finland, Czech Republic, Poland, Japan, Germany, UK and USA. He has been a PI, Co-investigator, Coordinator, Champion, or Scientific advisor of projects with budgets ranges of US$18,000 to US$6.2million. For details, see the list of on-going and past projects below.

Dr. Tugume’s research group works on plant-pathogen interactions, associated biotic stresses and disease management on crop commodities sweetpotato, cassava, banana, watermelons, and pumpkins. They focus on virus ecology, evolution, diagnostics, vector transmission, agro-ecosystem disease dynamics in these crops, and banana xanthomonas wilt pathosystem evolution. In addition, he is interested in sexual reproductive development of vegetatively propagated crops (banana, cassava and sweetpotato). For details, please see the list of publications below.

Dr. Tugume has over 30 scientific publications in international peer-reviewed journals generated in 12 years (2008-2022). He is an author, reviewer, and an editor. He is has supervised, mentored and/or examined 10 PhDs, 12 MSc, and over 30 BSc students’ research work. He has kin interest in science leadership and is fond of raising and motivating early career scientists, which is reminiscent of his broad reach in the crop commodities and subject themes of plant pathology, virology and sexual and vegetative reproductive development. Lately, he is at the helm of designing recommendations to Uganda government of Uganda on strategies for integrating science, technology and innovations (STIs) as a catalyst for national economic transformation and development.

Education

  • PhD in Plant Pathology from the University of Helsinki, Finland (2010)
  • M.Sc in Agriculture (Crop Science) (2003), from Makerere University
  • B.Sc (Botany and Biochemistry) (1999), from Makerere University.

Projects

PROJECT TITLE #1

Improving scalable banana agronomy for small-scale farmers in highland banana cropping systems in East Africa.

PROJECT LIFE:

2016-2021

PROJECT PI, Co-PI AND OTHER TEAM MAMBERS:

Dr. Jerome Kubiriba (PI), National Agricultural Research Organization (NARO)

Assoc. Prof. Arthur Tugume (Makerere University-based PI)

COLLABORATORS/COLLABORATING INSTITUTIONS:

International Institute of Tropical Agriculture (IITA)

Bioversity International-CIAT Alliance

Tanzania Agricultural Research Institute (TARI)

CABI International

African Soil Health Consortium (ASH-C)

BUDGET:

USD 5,700,000.00

PROJECT SUMMARY:

The significance of banana in the livelihoods of people in east and central Africa accounts for close to 4% of the GDP of the entire region. Most of the genotypes grown have emerged through centuries of farmer-assisted selection and are unique to the region. Wide yield gaps were previously observed because whereas the potential to yield and solve food shortages, many constraints accounted for failure to reach banana’s yield potential. The constraints included pests and diseases, drought spells and soil infertility. This project attempted a holistic approach to the banana production chain including marketing. Makerere University’s workstream however focused on redefining the contributions of banana xanthomonas wilt (BXW) as the main biotic constraint to banana productivity. BXW is caused by the bacterium Xanthomonas campestris musacearum (Xcm). The study also assessed the incidence of latent Xcm infections and promotion of carefully crafted cultural practices to enhance escape of banana plantations from further BXW-based damage in infested fields. This project was funded by the Bill & Melinda Gates Foundation (BMGF).

 

PROJECT TITLE #2

Transmission biology of the ipomovirus Sweet potato mild mottle virus in sweetpotato.

PROJECT LIFE:

2011-2013

PROJECT PI, Co-PI AND OTHER TEAM MAMBERS:

Assoc. Prof. Arthur Tugume (PI)

COLLABORATORS/COLLABORATING INSTITUTIONS:

Dr. Christopher Omongo (NaCRRI, Namulonge, Uganda)

BUDGET:

USD 60,000.00

PROJECT SUMMARY:

Sweetpotato is an important crop in Uganda but its contributions in food and income security is challenged by virus diseases. Notably, the virus named Sweet potato mild mottle virus (SPMMV) causes more severe damages when co-infecting with another unrelated virus, Sweet potato chlorotic stunt virus (SPCSV). While the epidemiology of SPCSV is fairly studied, SPMMV epidemiology is less studied including lack of knowledge of vectors transmitting the virus. To control disease complexes like those arising from multiple infections with sweetpotato viruses, it is essential that the epidemiology of viruses in mixed and single infections be studied. This study therefore investigated SPMMV epidemiology at field-level by analyzing the preferences of different insect species in respect to SPMMV infection in the field of host preferences of different insects in sweetpotato. This project was funded by Regional Universities FORUM for capacity building in Agriculture (RUFORUM).

 

PROJECT TITLE #3

Creation of an Undergraduate Bachelor of Science (BSc) in Biotechnology at Makerere University

PROJECT LIFE:

2008-2012

PROJECT PI, Co-PI AND OTHER TEAM MAMBERS:

Prof. Silvester Nyakaana (PI), Makerere University, Uganda

Prof. Hanington Oryem-Origa (Co-PI), Makerere University, Uganda

Assoc. Prof. Arthur Tugume (Co-PI), Makerere University, Uganda

Prof. Maud Kamatenesi-Mugisha (Co-PI), Makerere University, Uganda

Assoc. Prof. Gerald Mutumba (Co-PI) , Makerere University, Uganda

COLLABORATORS/COLLABORATING INSTITUTIONS:

Prof. Jari PT. Valkonen, University of Helsinki, Finland

BUDGET: USD 2,500,000.00

PROJECT SUMMARY:

This project was a result of societal demands on the premise of using biology and living systems based technologies in the transformation of Uganda’s the national economy and her science, technology and innovations (STIs) ecosystem. As one of the identified tenets of economic transformation, no existing academic programs existed in Ugandan universities to train and equip students at undergraduate level in Biotechnology. Therefore, the BSc Biotechnology was designed for this purpose providing fundamental knowledge and skill in all areas of biotechnology that allows easy application and/or easy higher degree specialization in all areas. This academic program was funded by the Government of Uganda in partnership with the World Bank.

Publications

  • Tugume AK, Mbanzibwa DR, Alicai T, Omongo CA, Maruthi MN (2023) Endemism and reemergence potential of the ipomovirus Sweet potato mild mottle virus (family Potyviridae) in Eastern Africa: half a century of mystery. Phytobiomes Journal. https://doi.org/10.1094/PBIOMES-05-22-0031-RVW

 

  • Osinde C, Sakamoto W, Kajiya-Kanegae H, Sobhy IS, Tugume AK, Nsubuga AM, Galis I (2022) Identification of quantitative trait loci associated with sorghum susceptibility to Asian stem borer damage. Journal of Plant Interactions. https://doi.org/10.1080/17429145.2022.2153182.

 

  • Walusansa A, Nakavuma JL, Asiimwe S, Ssenku JE, Aruhomukama D, Sekulima T, Kafeero HM, Anywar G, Katuura E, Nabatanzi A, Musisi NL, Tugume AK, Kakudidi EK (2022). Medically important bacteria isolated from commercial herbal medicines in Kampala city indicate the need to enhance safety frameworks. Scientific Reports 12, 16647 (2022). https://doi.org/10.1038/s41598-022-21065-y

 

  • Masika FB, Lequime S, Alicai T, Athman SY, Ipulet P, Ddamulira G, Tugume AK (2022) First report of Pepo aphid-borne yellows virus infecting watermelon (Citrullus lanatus) in Uganda. New Disease Reports, 46, e12110. https://doi.org/10.1002/ndr2.12110

 

  • Mustafa AS. Ssenku JE, Ssemanda P, Ntambi S, Dinesh-Kumar SP, Tugume AK (2022). Sandwich Enzyme-Linked Immunosorbent Assay for Quantification of Callose. Methods Protocols, 5, 54. https://doi.org/10.3390/mps5040054

 

  • Waniale A, Mukasa SB, Tugume AK, Kubiriba J, Tushemereirwe WK, Tumuhimbise R (2022) Early withering of enlarged ovules in pollinated fruits of bananas (Musa spp.) suggest abortion after fertilization. Horticulturae 2022, 8, 426. https://doi.org/10.3390/horticulturae8050426

 

  • Masika FB, Alicai T, Shimelis H, Ddamulira G, Athman SY, Ipulet P, Andama M, Tugume AK (2022). Pumpkin and watermelon production constraints and management practices in Uganda. CABI Agriculture and Bioscience, 3, 39. https://doi.org/10.1186/s43170-022-00101-x

 

  • Walusansa A, Asiimwe, Nakavuma JL, Ssenku JE, Katuura E, Kafeero HM, Aruhomukama DA, Nabatanzi AN, Anywar G, Tugume AK, Kakudidi EK (2022) Antibiotic-resistance in medically important bacteria isolated from commercial herbal medicines in Africa from 2000 to 2021: a systematic review and meta-analysis. Antimicrobial Resistance and Infection Control 11(11). https://doi.org/10.1186/s13756-022-01054-6

 

  • Mustafa AS, Tugume B, Ssenku JE, Ssemanda P, Athman SY, Oryem-Origa H, Kubiriba J, Savithramma D-K, Tugume AK (2022) Xanthomonas campestris musacearum bacterial infection induces organ-specific callose and hydrogen peroxide production in banana. PhytoFrontiers 2(3):202-2017. https://doi.org/10.1094/PHYTOFR-11-21-0073-R

 

  • Waniale A, Mukasa SB, Tugume AK, Tumuhimbise R, Kubiriba J, Tushemereirwe WK, Uwimana B, Gram G, Amah D, Swennen R (2021). Use of timelapse photography to determine flower opening time and pattern in banana (Musa) for efficient hand pollination. Scientific Reports, 11, 19480. https://doi.org/10.1038/s41598-021-98500-z

 

  • Waniale A, Swennen R, Mukasa SB, Tugume AK, Kubiriba J, Tushemereirwe WK, Batte M, Brown A, Tumuhimbise R (2021) Seed set patterns in East African Highland Cooking bananas are dependent on weather before, during and after pollination. Horticulturae 7(7), 165. https://doi.org/10.3390/horticulturae7070165.

 

  • Waniale A, Swennen R, Mukasa SB, Tugume AK, Kubiriba J, Tushemereirwe WK, Amah D, Tumuhimbise R (2021) Application of pollen germination media on stigmas during pollination increases seed set in East African Highland Cooking Bananas (Musa). Agronomy 11(6): 1085. https://doi.org/10.3390/agronomy11061085.

 

  • Waniale A, Swennen R, Mukasa SB, Tugume AK, Kubiriba J, Tushemereirwe WK, Batte M, Brown A, Tumuhimbise R (2021) Seed set patterns in East African Highland Cooking bananas show asymmetric distribution in bunches and fruits. Agronomy 11(4): 763. https://doi.org/10.3390/agronomy11040763.

 

  • Buttibwa M, Kawuki RS, Baguma JK, Nalela P, Eyokia M, Oshaba B, Ceballos H, Lentini Z, Baguma Y, Tugume AK (2020). Effect of self-pollination with heat-treated pollen on parthenocarpy and homozygosity in cassava. African Crop Science Journal 28(3): 449-472. DOI: https://dx.doi.org/10.4314/acsj.v28i3.9.

 

  • Buttibwa M Kawuki RS, Oshaba B, Eyokia M, Hershey C, Perera PIP, Heberle-Bors E, Baguma Y, Tugume AK (2020) In vitro culture of heat-treated anthers induces embryogenic callus in Cassava (Manihot esculenta Crantz). Journal of Plant Biochemistry and Physiology, 8:249. http://doi.org/10.35248/2329-9029.20.8.249.

 

  • Waniale A, Mukasa SB, Tugume AK, Tumuhimbise R, Kubiriba J, Swennen R (2020). Glucose performs better than fructose, sucrose, and diluted nectar for germination of banana pollen. Acta Horticulturae, 1282. https://doi.org/10.17660/ActaHortic.2020.1282.40

 

  • Baguma JK, Mukasa SB, Kawuki R, Tugume AK, Buttibwa M, Nalela P, Eyokia M, Oshaba B, Ceballos H, Lentini Z, Baguma Y (2019). Fruit set and plant regeneration in cassava following interspecific pollination with castor bean. African Crop Science Journal 27(1):99-119. DOI: https://dx.doi.org/10.4314/acsj.v27i1.8

 

  • Masika FB, Kisekka R, Alicai T, Tugume AK (2017) Incidence of viruses and virus-like diseases of watermelons and pumpkins in Uganda, a hitherto none-investigated pathosystem. African Journal of Agricultural Research, 12(3): 177-191. https://doi.org/10.5897/AJAR2016.11463

 

  • Tugume AK, Mukasa SB, Valkonen JPT (2016) Mixed Infections of four viruses, the incidence and phylogenetic relationships of Sweet potato chlorotic fleck virus (Betaflexiviridae) isolates in wild species and sweetpotatoes in Uganda and evidence of distinct isolates in East Africa. PLoS ONE 11(12): e0167769. https://doi.org/10.1371/journal.pone.0167769

 

  • Tugume AK, Mukasa SB, Valkonen JPT (2016) Transmission of viruses from Sweetpotatoes and Wild Species of Convolvulaceae in East Africa: Many Gaps to Fill. In: Vector-Mediated Transmission of Plant Pathogens. J.K. Brown (ed.). APS Press, American Phytopathological Society, USA. pp. 447-452. https://doi.org/10.1094/9780890545355.033

 

  • Buttibwa M, Kawuki RS, Tugume AK, Akol J, Magambo S, Apio H, Heberle-Bors E, Wedzony M, Ceballos H, Hershey C, Baguma Y (2015). In vitro embryo rescue and plant regeneration following self-pollination with irradiated pollen in Cassava (Manihot esculenta Crantz). African Journal of Biotechnology, 14(27): 2191-2201. https://doi.org/10.5897/AJB2015.14577

 

  • Mbanzibwa DR, Tugume AK, Chiunga E, Mark D, Tairo FD (2014) Small RNA deep sequencing-based detection and further evidence of DNA viruses infecting sweetpotato plants in Tanzania. Annals of Applied Biology, 165(3): 329-339. https://doi.org/10.1111/aab.12136

 

  • Tugume AK, Amayo R, Weinheimer I, Mukasa SB, Rubaihayo PR, Valkonen JPT (2013) Genetic variability and evolutionary implications of RNA silencing suppressor genes in RNA1 of Sweet potato chlorotic stunt virus isolates infecting sweetpotato and related wild species. PLoS ONE 8(11): e81479. https://doi.org/10.1371/journal.pone.0081479

 

  • Sun B.-J, Sun L.-Y, Tugume AK, Adams MJ, Yang J, Xie L.-H, Chen J.-P (2013) Selection pressure and founder effects constrain genetic variation in differentiated populations of a soilborne bymovirus Wheat yellow mosaic virus (Potyviridae) in China. Phytopathology 103: 949–959. https://doi.org/10.1094/PHYTO-01-13-0013-R

 

  • Kashif M, Pietilä S, Artola K, Jones RAC, Tugume AK, Mäkinen V, Valkonen JPT (2012). Detection of Viruses in Sweetpotatoes from Honduras and Guatemala Augmented by Deep-Sequencing of small-RNAs. Plant Disease 96:1430–1437. https://doi.org/10.1094/PDIS-03-12-0268-RE

 

  • Bi Y, Tugume AK, Valkonen JPT (2012). Small-RNA Deep-sequencing reveals Arctium tomentosum as a natural host of Alstroemeria virus X and a new putative Emaravirus. PLoS ONE 7(8): e42758. https://doi.org/10.1371/journal.pone.0042758

 

  • Clark CA, Davis JA, Abad JA, Cuellar W, Fuentes S, Kreuze J, Gibson R, Mukasa SB, Tugume AK, Tairo F, Valkonen JPT (2012). Sweetpotato viruses: 15 years of progress on understanding and managing complex diseases. Plant Disease 96:168–185. https://doi.org/10.1094/PDIS-07-11-0550

 

  • Mbanzibwa DR, Tian YP, Tugume AK, Patil BL, Yadav JS, Bagewadi B, Abarshi MM, Alicai T, Changadeya W, Mkumbira J, Muli MB, Mukasa SB, Tairo F, Baguma Y, Kyamanywa S, Kullaya A, Maruthi MN, Fauquet CM, Valkonen JPT (2011). Evolution of cassava brown streak disease-associated viruses. Journal of General Virology 92:974–987. https://doi.org/10.1099/vir.0.026922-0

 

  • Mbanzibwa DR, Tian YP, Tugume AK, Mukasa SB, Tairo F, Kyamanywa S, Kullaya A, Valkonen JPT (2011). Simultaneous virus-specific detection of the two cassava brown streak-associated viruses by RT-PCR reveals wide distribution in East Africa, mixed infections, and infections in Manihot glaziovii. Journal of Virological Methods 171:394–400. https://doi.org/10.1016/j.jviromet.2010.09.024

 

  • Tugume AK, Cuéllar WJ, Mukasa SB, Valkonen JPT (2010). Molecular genetic analysis of virus isolates from wild and cultivated plants demonstrates that East Africa is a hotspot for the evolution and diversification of Sweet potato feathery mottle virus. Molecular Ecology 19:3139–3156. https://doi.org/10.1111/j.1365-294X.2010.04682.x

 

  • Tugume AK, Mukasa SB, Kalkkinen N, Valkonen JPT (2010). Recombination and selection pressure in the ipomovirus Sweet potato mild mottle virus (Potyviridae) in wild species and cultivated sweetpotato in the centre of evolution in East Africa. Journal of General Virology 91:1092–1108. https://doi.org/10.1099/vir.0.016089-0

 

  • Mbanzibwa DR, Tian YP, Tugume AK, Mukasa SB, Tairo F, Kyamanywa S, Kullaya A, Valkonen JPT (2009). Genetically distinct strains of Cassava brown streak virus in the Lake Victoria basin and the Indian Ocean coastal areas of East Africa. Archives of Virology 154:353–359. https://doi.org/10.1007/s00705-008-0301-9

 

  • Kallinen AK, Lindberg IL, Tugume AK, Valkonen JPT (2009). Detection, distribution, and genetic variability of European mountain ash ringspot-associated virus. Phytopathology 99:344–352. https://doi.org/10.1094/PHYTO-99-4-0344

 

  • Tugume AK, Mukasa SB, Valkonen JPT (2008). Natural wild hosts of Sweet potato feathery mottle virus show spatial differences in virus incidence and virus-like diseases in Uganda. Phytopathology 98:640–652. https://doi.org/10.1094/PHYTO-98-6-0640